Strain Optimization

Gram negative bacteria are the standard when it comes to protein expression. However, these engineered strains and plasmids are often the result of selection and unknown mutations that allow them to grow faster. In addition many common lab and production strains contain genes that could cause detrimental outcomes; such as prophage islands, virulence genes, and elongated lipopolysaccharides (LPS or endotoxins).

T3S utilizes proven and reliable genetic engineering and selection processes to make large deletions or insertions of genetic regions to benefit production outcomes.

In addition, we also utilize engineered and natural promoters into the bacterial chromosome to tightly regulate gene expression, provide high transcription and translation yield, strain stability, and utilize in expensive induction methods.

Please contact us if you would like to discuss our strain development services.